CD4 Lymphocytes

Label Mnemonic:	CD4/3
Epic code:	LAB1280
Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	86361

Specimen(s):

Peripheral blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Alternate Collection Media:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Adult: 3 mL whole blood (Lavender) or 6 mL whole blood (Pink)

Pediatric: 2 mL whole blood

Testing Schedule:

0800-1630 Monday through Friday, excluding UIHC designated holidays. Must be received by 1615 before weekends or holidays.

Turn Around Time:

2 days

Reference Range:

Adult reference ranges for whole blood lysis method by flow 
cytometry:

T Cells (CD4)     34-62%     Absolute Counts:  263-2045/mm3

CD3 test is run as an internal quality assurance measure as directed 
by CDC guidelines. The results of this QA will not be charged.

Pediatric reference ranges will be provided.

Comments:

For further details, refer to How to Send Flow Cytometry Specimens.

Please print, complete and submit the Advance Beneficiary 
Notice (ABN) along with the Comprehensive Hematopathology 
Requisition before shipping the specimen.  Include pertinent clinical 
information on the requisition.  Recent corticosteroid or chemotherapy 
may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

Instructions:

Maintain sample at room temperature; do not incubate or refrigerate. Specimen is only viable for 48 hours at room temperature.

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.

Sample Storage:

Room Temperature.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.