CBC with Automated Differential
Label Mnemonic: CBC, DIFF
Epic code: LAB293
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 85025
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Full draw; 3.0 mL lavender top; (or fingerstick-purple top microtainer®)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
  Adults Children (5-18 yrs)
  (mm3) (mm3)
Band Neutrophils - -
Neutrophils 2188-7800 1700-7500
Eosinophils 40-390 40-650
Basophils 10-136 7-140
Monocytes 130-860 28-825
Lymphocytes 875-3300 1250-7000
Immature Granulocytes - -
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.

A CBC must be ordered to perform a differential. Hematology instruments are automated differential screening instruments that identify WBC's by their size and staining activity. It performs a complete cell identification that includes neutrophils, lymphocytes, monocytes, basophils, eosinophils and immature granulocytes. The automated instruments count 32,000 white blood cells and statistically provide a more accurate differential count than a manual differential. All differentials will be analyzed on the instrument first. If the results fail the screening criteria, a manual differential will be performed. Pathologist approval is required to perform a manual differential on a specimen with an automated differential that passed the screening criteria.

Cases with significant abnormal cells may require a differential with narrative description and interpretation by the pathologist. In these cases, results will be accompanied by the pathologist's report and charge.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.
Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain
Sample Processing:
Submit whole blood in original container. DO NOT CENTRIFUGE.
Sample Storage:
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code: