Variants with Established Clinical Significance (Tier 1), with Potential Clinical Significance (Tier 2) and/or with Uncertain Clinical Significance (Tier 3) are reported from the 507 gene regions interrogated by the assay and that are known to be somatically altered in human cancers including some that are validated targets for therapy, either approved or in clinical trials, and or are drivers of oncogenesis. Benign and Likely Benign variants are not included but are available upon request. Sequencing includes targeted or full coding sequence depending on the gene and the spectrum of sequence variants includes substitutions, insertions, deletions (up to 42 base pairs) and copy number alterations (gene deletion or gene amplification). Additionally, biomarker information for microsatellite instability (MSI) and tumor mutation burden (TMB) are reported.

Gene List:

ABL1, ABL2, ACVR1B, AKT1*, AKT2*, AKT3*, ALK*, AMER1*, APC*, AR*, ARAF, ARFRP1, ARID1A, ARID1B, ARID2, ASXL1, ATM, ATR, ATRX*, AURKA*, AURKB, AXIN1, AXIN2, AXL*, B2M, BAP1, BARD1, BCL10, BCL11B, BCL2, BCL2L1, BCL2L2, BCL6*, BCL7A, BCOR*, BCORL1, BCR, BIRC3, BLM, BMPR1A, BRAF*, BRCA1, BRCA2*, BRCC3, BRD3, BRD4, BRIP1, BTG1, BTK, BUB1B, C11orf30 (EMSY)*, CALR, CARD11*, CASP8, CBFB, CBL, CBLB, CBLC, CCND1*, CCND2*, CCND3*, CCNE1*, CD19, CD274, CD28, CD40, CD58, CD79A, CD79B, CDC73, CDH1*, CDK12, CDK4*, CDK6*, CDK8, CDKN1A, CDKN1B*, CDKN2A*, CDKN2B*, CDKN2C, CEBPA, CHD2, CHD4*, CHEK1, CHEK2, CIC, CIITA, COL1A1, CREBBP, CRKL, CRLF2, CSF1R, CSF3R, CSNK1A1, CTCF, CTLA4, CTNNA1, CTNNB1, CUL3, CUX1, CXCR4, CYLD, DAXX*, DDR2*, DDX3X*, DDX41, DICER1, DNM2, DNMT3A, DOT1L, EBF1, EED, EFNB2, EGFR*, EGR2, EIF1AX, EP300, EPCAM, EPHA3, EPHA5, EPHA7, EPHB1, EPHB4, ERBB2*, ERBB3*, ERBB4, ERCC1, ERCC2, ERCC3, ERCC4, ERCC5, ERG, ERRFI1, ESR1*, ETNK1, ETS1, ETV1, ETV4, ETV5, ETV6, EWSR1, EXT1, EZH2*, FAM175A (ABRAXAS1), FAM46C (TENT5C), FAM5C (BRINP3), FANCA*, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCL, FANCM, FAS*, FAT1*, FAT3, FBXO11, FBXW7, FGF10, FGF14, FGF19*, FGF23*, FGF3*, FGF4*, FGF6, FGFR1*, FGFR2*, FGFR3*, FGFR4*, FH, FLCN, FLT1, FLT3*, FLT4*, FOXA1*, FOXL2, FOXO1, FOXP1, FOXR2, FRS2, FUBP1, FUS, GABRA6, GATA1, GATA2*, GATA3, GATA4, GATA6, GEN1, GID4, GLI1, GNA11, GNA13, GNAQ, GNAS*, GNB1, GPC3, GPR124 (ADGRA2), GREM1, GRIN2A, GRM3, GSK3B, H3F3A (H3-3A), HDAC4, HGF, HIF1A, HIST1H1E (H1-4), HIST1H3B (H3C2), HIST1H3C (H3C3), HLA-A, HLA-B, HLA-DRB1, HMGA2, HNF1A, HOXB13, HRAS, HSD3B1, HSP90AA1, ID3, IDH1, IDH2, IGF1R*, IGF2, IKBKE, IKZF1, IKZF2, IKZF3, IL2RG, IL7R*, INHBA, INPP4B, IRF2, IRF4, IRF8, IRS2, ITK, ITPKB, JAK1, JAK2, JAK3, JAZF1, JUN, KAT6A, KDM5A, KDM5C*, KDM6A*, KDR*, KEAP1, KEL, KIF1B, KIF5B, KIT*, KLF2, KLF4, KLHL6, KMT2A, KMT2B, KMT2C, KMT2D, KRAS*, LIFR, LMO1, LRP1B, LYN, LYST, LZTR1, MAGI2, MAML2, MAP2K1, MAP2K2, MAP2K4*, MAP3K1*, MAP3K3, MAP3K7, MAPK1, MAX, MCL1*, MDM2*, MDM4*, MECOM*, MED12, MEF2B*, MEN1, MET*, MITF*, MLH1, MLH3, MN1, MPL*, MRE11A (MRE11), MSH2, MSH3, MSH6, MTOR, MUTYH, MYB, MYBL1, MYC*, MYCL*, MYCN*, MYD88, MYH9, NBN, NCOA2, NCOR1, NF1*, NF2*, NFE2, NFE2L2, NFKBIA, NFKBIE, NKX2-1, NOTCH1, NOTCH2, NOTCH3, NPM1*, NRAS, NSD1, NT5C2, NTHL1, NTRK1*, NTRK2*, NTRK3*, NUP214, NUP93, NUTM1, PAK3, PALB2, PARK2 (PRKN), PAX3, PAX5, PAX7, PAX8, PBRM1, PDCD1, PDCD1LG2, PDGFB, PDGFRA*, PDGFRB*, PDK1, PHF6*, PHOX2B, PIGA, PIK3C2B*, PIK3CA*, PIK3CB*, PIK3CD, PIK3CG, PIK3R1, PIK3R2*, PIM1, PLAG1, PLCG1*, PLCG2, PML, PMS1, PMS2, POLD1, POLE, POLR2A, POT1, PPARG*, PPM1D, PPP2R1A, PRDM1*, PREX2, PRKAR1A, PRKCI, PRKDC, PRPF40B, PRPF8, PRSS8, PTCH1, PTEN*, PTPN11, PTPRD, PTPRT, QKI, RAB35, RAC1*, RAD21, RAD50, RAD51, RAD51B, RAD51C, RAD51D, RAF1*, RANBP2, RARA*, RASA1, RB1*, RBM10*, RECQL, RECQL4, REL, RELA, RET, RHOA, RICTOR*, RINT1, RIT1*, RNF43, ROS1*, RPS15, RPS20, RPTOR*, RRAGC, RSPO2, RSPO3, RUNX1, RUNX1T1, SAMD9, SAMD9L, SBDS, SDHA, SDHB, SDHC, SDHD, SETBP1, SETD2, SF1, SF3A1, SF3B1, SGK1, SH2B3, SLIT2, SLX4, SMAD2*, SMAD3, SMAD4*, SMARCA4, SMARCB1*, SMC1A, SMC3, SMO, SNCAIP, SOCS1, SOS1, SOX10, SOX2*, SOX9, SPEN, SPOP, SPTA1, SRC*, SRSF2, SS18, STAG2*, STAT3, STAT4, STAT5B, STAT6, STK11, SUFU, SUZ12, SYK, TAF1, TBL1XR1, TBX3, TCEB1 (ELOC), TCF12, TCF3, TCF7L2, TERC, TERT*, TET1, TET2, TFE3, TFEB, TGFBR2, TMPRSS2, TNFAIP3, TNFRSF14, TOP1*, TOP2A, TP53*, TP63*, TPMT, TRAF3, TRAF7, TSC1*, TSC2*, TSHR, TYK2, U2AF1, U2AF2, UBA1, USP6, VAV1, VEGFA, VHL, WHSC1 (NSD2), WISP3 (CCN6), WRN, WT1, XPC, XPO1, XRCC2, YAP1*, YWHAE, ZBTB2, ZMYM3*, ZNF217*

An asterisk (*) indicates that this gene is analyzed for copy number variations (CNVs).