TP53 Gene Analysis and Interpretation

Label Mnemonic:	TP53
Epic code:	LAB8967
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81351 81479 (Medicare Only)

Specimen(s):

Blood, Bone Marrow Aspirate, Tissue (formalin fixed paraffin embedded)

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Blood: Collect ONE full Pink top EDTA tube, requires a dedicated tube.

Bone Marrow Aspirate: Collect ONE full Pink top EDTA tube, requires a dedicated tube.

One tumor-containing formalin-fixed paraffin embedded tissue block or 10 unstained 4 micron-thick sections on non-plus slides and one H&E stained section from one tumor-containing FFPET block or any or all unstained or stained slides from tumor-containing, fine needle aspirate cytologic preparations. Specimen requires 20% or greater tumor cell content.

Rejection Criteria:

Tumor specimens containing less than 20% tumor cells may be unacceptable.

Testing Schedule:

Twice weekly

Turn Around Time:

7-10 days

Comments:

TP53 mutations are identified by performing full gene coding sequence analysis.

Methodology:

DNA sequencing by synthesis

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
Place requisition into outside pocket of bag.
Protect phlebotomy tube from excessive heat and ship in cooled container during summer months.

CPT Code:

81351
81479 (Medicare Only)