NTRK1, 2 and 3 Fusion with Interpretation
Label Mnemonic: NTRKT
Epic code: LAB8826
Order form: Molecular Oncology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 81194
Formalin-fixed, paraffin embedded tissue or cytology fine needle aspirate smears; 2000 tumor cells minimum requirement, or >20% tumor cells from tissue sections
Formalin-fixed, paraffin embedded tissue block, or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section.
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around Time:
10-14 days upon receipt in laboratory
Reference Range:
Fusion or mutation not detected
The panel targets gene rearrangements of the NTRK1, NTRK2 and NTRK3. NTRK gene rearrangements represent the fusion between NTRK1, NTRK2, or NTRK3 with various partner genes that result in constitutive kinase activity.

NTRK gene rearrangements are found at high frequencies in rare cancer types including secretory carcinoma of the breast and salivary gland, congenital or infantile fibrosarcoma and at lower frequencies (commonly  <1%) in a range of other tumor types.

The FDA granted approval for use of larotrectinib for adult and pediatric patients with solid tumors that have NTRK gene rearrangements that are either metastatic or non-resectable, who have no satisfactory alternative treatments or whose cancer has progressed following treatment (based on data from the LOXO-TRK-14001 (NCT02122913), SCOUT (NCT02637687), and NAVIGATE (NCT02576431) clinical trials).

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Total nucleic acid including RNA is isolated from tumor specimens. Reverse transcription, anchored multiplexed PCR for targeted next- generation sequencing is performed.
Sample Storage:
Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship at room temperature.
Protect block or slides from excessive heat and ship in cooled container during summer months.
CPT Code: