NPM1 Mutation Quantitative, MRD Monitoring and Interpretation

Label Mnemonic:	NPM1BMQNT
Epic code:	LAB8829
Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81310

Specimen(s):

Bone Marrow Aspirate

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

5.0 mL Bone Marrow Aspirate in EDTA anti-coagulated pink top tube

Turn Around Time:

10-14 days upon receipt in laboratory

Comments:

Methodology:

The NPM1 Mutation Quantitation assay uses multiplex reverse transcription in combination with droplet digital PCR technology. The type A NPM1 duplications occur in most AML cases. The assay can reliably detect 1 tumor cell in a background of 20,000 normal cells. The limit of quantitation is 0.005% NPM1/ABL1 ratio.

Instructions:

Testing requires dedicated collection tube.

Sample Processing:

Do Not Centrifuge.

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place packing list into outside pocket of bag.
Protect phlebotomy tube from excessive heat and ship in cooled container during summer months.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.

CPT Code:

81310