NPM1 Mutation Quantitative, MRD Monitoring and Interpretation

Label Mnemonic:	NPM1QNT
Epic code:	LAB8828
Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81310

Specimen(s):

Whole Blood

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

5.0 mL Whole Blood in EDTA anti-coagulated pink top tube

Rejection Criteria:

Specimens must be received within 72 hours of collection. Optimal results are achieved if specimen is received within 48 hours of collection.

Turn Around Time:

10–14 days upon receipt in laboratory

Comments:

Test Limitations:

Samples must be refrigerated within one hour and must be received within 72 hours of collection. Optimal results are achieved if specimen is received within 48 hours of collection.

Methodology:

The NPM1 Mutation Quantitation assay uses multiplex reverse transcription in combination with droplet digital PCR technology. The type A NPM1 duplications occur in most AML cases. The assay can reliably detect 1 tumor cell in a background of 20,000 normal cells. The limit of quantitation is 0.005% NPM1/ABL1 ratio.

Instructions:

Testing requires dedicated collection tube.

Sample Processing:

Do Not Centrifuge.

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place packing list into outside pocket of bag.
Ship refrigerated.