Rapid Plasma Reagin (RPR) with Reflex Confirmation

Label Mnemonic:	RPRP
Epic code:	LAB8647
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	86592

Specimen(s):

Serum

Collection Medium:

BD Gold SST 5 mL Vacutainer

Minimum:

5 mL serum from a BD Gold SST tube

Turn Around Time:

1 day

Reference Range:

Negative

Comments:

This testing consists of RPR with reflex to titer and TP-PA confirmation. Additional charges apply if TP-PA confirmation is performed.

This is generally not the preferred test for syphilis screening but may be indicated in some clinical situations. Syphilis Total Antibodies with Reflex Confirmation (LAB8631) is recommended for routine syphilis testing.

Test Limitations:

The assay is unaffected by icterus < 66 mg/dL, hemolysis (Hb < 500 mg/dL), or lipemia < 2000 mg/dL). RPR assay interference resulting in false positive results may occur with cross-reacting antibodies from patients diagnosed with systemic lupus erythematosus, malaria, autoimmune disease, viral pneumonia, and people who have been recently vaccinated (including vaccines for SARS-CoV-2). RPR results should be interpreted in conjunction with patient history, risk factors, and other syphilis testing.

Methodology:

Multiplex flow immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Store frozen.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Ship frozen.

CPT Code:

86592