Chromogenic Factor VIII Assay
| Label Mnemonic: | CH8 |
| Epic code: | LAB8465 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 85130 |
Specimen(s):
Plasma
Collection Medium:
 |
| Light Blue top tube 2.7 mL (Na Citrate) |
Minimum:
Full draw; 2.7 mL light blue top
Rejection Criteria: Short drawn tube, clot, traumatic tap (excessive hemolysis).
Turn Around
Time:
24–48 hours
Reference Range:
43–159%
Methodology:
Chromogenic
Sample
Processing:
Double-centrifuge specimen to prepare platelet-free plasma.
Immediately centrifuge specimen at 3500 RPM for 12 minutes.
Using a plastic transfer pipette, carefully remove plasma from cells avoiding the platelet/buffy coat.
Place plasma into a labeled plastic tube.
Centrifuge plasma again at 3500 RPM for 12 minutes.
Remove the top of the plasma and place into a labeled plastic tube.
Freeze double-spun plasma within 4 hours of collection time at -20 degrees C or below.
Immediately centrifuge specimen at 3500 RPM for 12 minutes.
Using a plastic transfer pipette, carefully remove plasma from cells avoiding the platelet/buffy coat.
Place plasma into a labeled plastic tube.
Centrifuge plasma again at 3500 RPM for 12 minutes.
Remove the top of the plasma and place into a labeled plastic tube.
Freeze double-spun plasma within 4 hours of collection time at -20 degrees C or below.
Sample
Storage:
Freeze specimen immediately.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Samples received thawed will be cancelled.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Samples received thawed will be cancelled.