JAK2 Reflex for Polycythemia with Interpretation
Label Mnemonic: | RFLX JAK2 PV |
Epic code: | LAB8540 |
Order form: | Comprehensive Hematopathology Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | The appropriate CPT code(s) will be billed depending on the patient's results including; 81270, and 81403 times 1 or times 2. |
Specimen(s):
Whole Blood
Collection Medium:
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Pink top tube 6 mL (K2-EDTA) |
Minimum:
Adults: 3 mL whole blood. Testing requires a dedicated collection
tube.
Testing Schedule:
Weekly
Turn Around Time:
7-10 working days
Reference Range:
Mutation not detected
Comments:
By ordering this test the physician directs the molecular pathology
laboratory to perform testing reflexively for the mutations commonly
associated with polycythemia vera. JAK2 exons 12-14 and MPL exon 10
coding sequences are amplified by polymerase chain reaction (PCR)
and sequenced in both directions using next generation sequencing.
The JAK2 V617F mutation, or mutations in JAK2 Exons 12-14, and MPL
exon 10 are reported in that order; once a mutation is identified no
further analysis is performed (See the
Polycythemia vera Algorithm).
The reference sequences with which this patient's DNA sequence are
compared are NM_004972, and NM_005373.1.
Methodology:
Massively parallel next generation sequencing on DNA
Sample Processing:
Label transport tube with two patient identifiers, date and time of
collection.
Submit whole blood in original container. DO NOT CENTRIFUGE.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Submit whole blood in original container. DO NOT CENTRIFUGE.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Sample Storage:
Room temperature for up to 24 hours, then refrigerate the whole
blood if it is necessary to be held overnight, weekends, or
holidays.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship refrigerated.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend early AM overnight shipping or equivalent if not on courier service.
Place requisition into outside pocket of bag.
Ship refrigerated.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend early AM overnight shipping or equivalent if not on courier service.