MYD88 Exon 5 Sequencing
Label Mnemonic: | MYD88, MYD88BL, MYD8 |
Epic code: |
LAB8392 (FFPE Tissue) LAB8462 (Blood) LAB8463 (Marrow) |
Order form: | Comprehensive Hematopathology Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 81305 |
Specimen(s):
FFPE Tissue, Peripheral Blood, Bone Marrow Aspirate
Formalin Fixed, Parafin Embedded Tissue Block
10 unstained 5-10 μM thick slides at 20-25°C and an H&E slide of the tissue or clot.
Formalin Fixed, Parafin Embedded Tissue Block
10 unstained 5-10 μM thick slides at 20-25°C and an H&E slide of the tissue or clot.
Collection Medium:
Pink top tube 6 mL (K2-EDTA) |
Minimum:
Collect 5-10 mL Peripheral Blood in EDTA (in TWO Pink tubes)
Testing requires a dedicated tube.
Collect 3-5 mL Bone Marrow Aspirate in EDTA Pink top tube)
Testing requires a dedicated tube.
Absolute Minimum: 1 mL Bone Marrow
Tissue (fresh or formalin fixed paraffin embedded)
Testing requires a dedicated tube.
Collect 3-5 mL Bone Marrow Aspirate in EDTA Pink top tube)
Testing requires a dedicated tube.
Absolute Minimum: 1 mL Bone Marrow
Tissue (fresh or formalin fixed paraffin embedded)
Rejection Criteria:
Frozen, clotted or severely hemolyzed specimens are NOT acceptable.
Specimens that have been decalcified or fixed in B5 fixative will
not be accepted. Tumor specimens with inadequate cellular material
or a tumor percentage will be rejected.
Turn Around Time:
6-10 days
Comments:
Myeloid differentiation primary response 88 (MYD88) encodes a
cytosolic adapter protein that is frequently mutated in Waldenström
macroglobulinemia (WM) and lymphoplasmacytic lymphoma (LPL).
Approximately 90% of cases harbor a leucine to proline mutation at
codon 265 (p.L265P, c.794T>C) in exon 5. Detection of MYD88 L265P
can help establish a diagnosis of LPL in patients with typical
histologic and flow cytometry findings as it rarely occurs in
marginal zone lymphoma or chronic lymphocytic leukemia. MYD88
mutation status may also assist with risk stratification of IgM
monoclonal gammopathy of undetermined significance or MGUS as
patients who have the mutation are possibly at increased risk of
progression to WM or LPL.
Methodology:
Massively parallel Next Generation DNA sequencing by synthesis,
semiconductor technology, Ion Personal Genomics Machine
Transport Instructions:
Place specimen into Styrofoam container, seal container.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.