Calreticulin Mutation Analysis with Interpretation
Label Mnemonic: CALRBM
Epic code: LAB8077
Order form: Comprehensive Hematopathology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 81219
Bone Marrow Aspirate
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Adults 3 mL bone marrow aspirate. Testing on smaller volumes than those requested will be attempted. However, in some cases, small bone marrow volumes may compromise the ability to perform testing. Testing requires a dedicated collection tube.
Testing Schedule:
Turn Around Time:
7 working days
Reference Range:
No mutations detected.
Calreticulin (CALR) mutations can be used to help identify patients with myeloproliferative neoplasms, in particular essential thrombocythemia and primary myelofibrosis. The bulk of the mutations that have been described are present in ex.9. The two most common mutations are a 52 bp deletion, c.1099_1150del, and a 5 bp insertion, c.1154_1155insTTGTC, though rare missense and insertion/deletion mutations have been described.
PCR amplification and fragment separation by capillary electrophoresis.
Sample Processing:
Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Do Not Centrifuge.
Sample Storage:
Room temperature for up to 24 hours, then refrigerate the bone marrow if it is necessary to be held overnight, or weekends, or holidays.
Transport Instructions:
Place requisition into outside pocket of bag.
Ship refrigerated.
CPT Code: