Cancer Mutation Profiling and Interpretation

Label Mnemonic:	CMPBM
Epic code:	LAB8094
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81445

Specimen(s):

Bone Marrow Aspirate

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

2 mL Bone Marrow Aspirate in EDTA (pink tube).

Rejection Criteria:

Specimens fixed in B5 fixative or decalcified will not be accepted. Tumor specimens containing less than 20% tumor cells may be unacceptable.

Testing Schedule:

Weekly

Turn Around Time:

21 days

Comments:

The Cancer Mutation Profiling test is a massively parallel next generation DNA sequencing-based assay that targets commonly mutated or hotspot regions of 50 cancer-related genes.

Genes Assessed: ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARC1, SMO, SRC, STK11, TP53, and VHL.

Methodology:

Massively parallel Next Generation DNA sequencing by synthesis, semiconductor technology, Ion Torrent

Instructions:

Testing requires dedicated collection tube.

Sample Storage:

Refrigerate.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship refrigerated.
Place requisition into outside pocket of bag.
Protect phlebotomy tube from excessive heat and ship in cooled container during summer months.