Mycobacteria Tuberculosis PCR with Rifampin Resistance (includes culture and stain)

Label Mnemonic:	TBPCR
Epic code:	LAB7618
Order form:	Microbiology Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	87556

Specimen(s):

Sputum, Induced Sputum, Bronchoalveolar Lavage or Bronchial Washings

Collection Medium:

Sterile container

Minimum:

5-15 mL in a sterile container. Pooled specimens are unacceptable.

Rejection Criteria:

More than 1 specimen/day or 3 specimen/week.
Non-respiratory specimens. Repeat testing subsequent to a positive test.

Testing Schedule:

0700-1630, 7 days a week, including holidays.

Turn Around Time:

24 hours (upon receipt in laboratory)

Comments:

The Xpert MTB/RIF Assay is intended for use with specimens from patients for whom there is clinical suspicion of tuberculosis and who have received no therapy or less than 3 days of antituberculosis therapy. This test does not provide confirmation of rifampin susceptibility since mechanisms of rifampin resistance other than those detected by this device may exist. The Xpert MTB/RIF Assay must be used in conjunction with a mycobacterial culture to address the risk of false negative results and to recover organisms for further testing and drug susceptibility testing.

This test is for diagnostic purposes and is not a test of cure. Repeat testing after initial positive test will require approval of the pathology resident on call or pager 4290.

Methodology:

PCR amplification: Xpert MTB/RIF Assay (Cepheid)

Sample Processing:

Label transport tube with two patient identifiers, date and time of collection.
Specimen should be collected and packaged as close to shipping time as possible.
If transport is delayed, store specimen at 2-8°C (stable for up to 7 days). Alternatively, specimens can be kept at room temperature (35°C) for up to 72 hours.

Transport Instructions:

Use refrigerated coolant packs.
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.