Mycobacteria Tuberculosis PCR with Rifampin Resistance (includes culture and stain)
| Label Mnemonic: | TBPCR |
| Epic code: | LAB7618 |
| Order form: | Microbiology Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 87556 |
Specimen(s):
Sputum, Induced Sputum, Bronchoalveolar Lavage or Bronchial Washings
Collection Medium:
| Sterile container |
Minimum:
5-15 mL in a sterile container.
Pooled specimens are unacceptable.
Rejection Criteria:
More than 1 specimen/day or 3 specimen/week.
Non-respiratory specimens. Repeat testing subsequent to a positive test.
Non-respiratory specimens. Repeat testing subsequent to a positive test.
Testing Schedule:
0700-1630, 7 days a week, including holidays.
Turn Around Time:
24 hours (upon receipt in laboratory)
Comments:
The Xpert MTB/RIF Assay is intended for use with specimens from patients for whom there is clinical suspicion of tuberculosis and who have received no therapy or less than 3 days of antituberculosis therapy. This test does not provide confirmation of rifampin susceptibility since mechanisms of rifampin resistance other than those detected by this device may exist. The Xpert MTB/RIF Assay must be used in conjunction with a mycobacterial culture to address the risk of false negative
results and to recover organisms for further testing and drug susceptibility testing.
This test is for diagnostic purposes and is not a test of cure. Repeat testing after initial positive test will require approval of the pathology resident on call or pager 4290.
This test is for diagnostic purposes and is not a test of cure. Repeat testing after initial positive test will require approval of the pathology resident on call or pager 4290.
Methodology:
PCR amplification: Xpert MTB/RIF Assay (Cepheid)
Sample Processing:
Label transport tube with two patient identifiers, date and time of collection.
Specimen should be collected and packaged as close to shipping time as possible.
If transport is delayed, store specimen at 2-8°C (stable for up to 7 days). Alternatively, specimens can be kept at room temperature (35°C) for up to 72 hours.
Specimen should be collected and packaged as close to shipping time as possible.
If transport is delayed, store specimen at 2-8°C (stable for up to 7 days). Alternatively, specimens can be kept at room temperature (35°C) for up to 72 hours.
Transport Instructions:
Use refrigerated coolant packs.
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.