KIT Mutation (D816V) For Mast Cell Disease
| Label Mnemonic: | KITMAST |
| Epic code: | LAB7567 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 81273 |
Specimen(s):
Whole Blood or Bone Marrow
Collection Medium:
|
| Pink top tube 6 mL (K2-EDTA) |
Minimum:
Whole Blood - Do Not Freeze
Preferred Minimum: 5 mL in pink top tube
Absolute Minimum: 1 mL in pink top tube
Bone Marrow - Do Not Freeze
Preferred Minimum: 3 mL in pink top tube
Absolute Minimum: 1 mL in pink top tube
Preferred Minimum: 5 mL in pink top tube
Absolute Minimum: 1 mL in pink top tube
Bone Marrow - Do Not Freeze
Preferred Minimum: 3 mL in pink top tube
Absolute Minimum: 1 mL in pink top tube
Rejection Criteria:
Plasma, serum, FFPE tissue blocks/slides, or fresh or frozen tissue,
DNA extracted by a non-CLIA certified lab. Specimens collected in
anticoagulants other than EDTA or sodium heparin. Clotted or grossly
hemolyzed specimens.
Testing Schedule:
DNA isolation: Sun-Sat
Assay: Varies
Assay: Varies
Turn Around Time:
2-7 days upon receipt at reference laboratory.
Interpretive Data:
Refer to report.
Methodology:
Droplet Digital Polymerase Chain Reaction
Sample Processing:
Pink tube is sent as whole blood, no processing necessary.
Label tube with two Full patient identifiers, date and time of collection.
Label tube with two Full patient identifiers, date and time of collection.
Sample Storage:
Refrigerate.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal
bag.
Place completed requisition into outside pocket of bag.
Place coolant packs on top of specimen in Styrofoam container to maintain refrigerated temperature.
Place completed requisition into outside pocket of bag.
Place coolant packs on top of specimen in Styrofoam container to maintain refrigerated temperature.
Zcode:
Z006N