Factor IX Assay

Label Mnemonic:	FC9
Epic code:	LAB308
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	85250

Specimen(s):

Plasma More information

Collection Medium:

Light Blue top tube 2.7 mL (Na Citrate)

Minimum:

Full draw; 2.7 mL light blue top

Rejection Criteria:

Short drawn tube, clot, traumatic tap (excessive hemolysis).

Testing Schedule:

0800-1630 Monday, Wednesday and Friday. If you need results STAT, call Hematology resident (0800-1630, M-F) at 356-8628. All other times page the Pathology resident on-call at 3404.

Reference Range:

60% - 177%

Methodology:

Optical clot detection.

Sample Processing:

Double-centrifuge specimen to prepare platelet-free plasma.
Immediately centrifuge specimen at 3500 RPM for 12 minutes.
Using a plastic transfer pipette, carefully remove plasma from cells avoiding the platelet/buffy coat.
Place plasma into a labeled plastic tube.
Centrifuge plasma again at 3500 RPM for 12 minutes.
Remove the top of the plasma and place into a labeled plastic tube.
Freeze double-spun plasma within 4 hours of collection time at -20 degrees C or below.

Sample Storage:

Freeze specimen immediately.

Transport Instructions:

Place dry ice on top of specimen in Styrofoam container.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Samples received thawed will be cancelled.

See Additional Information:
Hemolysis Chart