Staphylococcus aureus (MRSA/MSSA) by PCR
Label Mnemonic: | SAPCR |
Epic code: | LAB5810 |
Order form: | Microbiology/Molecular Infectious Disease Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 87641 |
Specimen(s):
Surveillance Swab Specimen collected from Nares
Rejection Criteria:
Specimen collected using different swab (not Copan dual swab) or
from site other than nares will be rejected.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
2 hours (upon receipt in laboratory)
Comments:
Used to detect colonization with SA and methicillin-resistant
Staphylococcus aureus (MRSA). A positive result does not necessarily
indicate viable organism.
The primers and probes in the Xpert SA Nasal Complete assay detects a proprietary sequence for the staphylococcal protein A (spa) gene, the gene for methicillin resistance (mecA), and the staphylococcal cassette chromosome mec (SCCmec) inserted into the SA chromosomal attB site. Inhibition of the SA Nasal Complete assay resulting in Invalid or false Negative test results has been observed in the presence of inhaled nasal steroids Flonase and Nasonex. Reliable results are dependent on proper specimen collection, handling, and storage.
The primers and probes in the Xpert SA Nasal Complete assay detects a proprietary sequence for the staphylococcal protein A (spa) gene, the gene for methicillin resistance (mecA), and the staphylococcal cassette chromosome mec (SCCmec) inserted into the SA chromosomal attB site. Inhibition of the SA Nasal Complete assay resulting in Invalid or false Negative test results has been observed in the presence of inhaled nasal steroids Flonase and Nasonex. Reliable results are dependent on proper specimen collection, handling, and storage.
Methodology:
PCR amplification; Xpert SA Test (Cepheid)
Instructions:
Copan Dual Swab (#26200) is available from Hospital Stores. With patient's head tilted back, insert both dry swabs (leave attached to red cap) approximately 1-2 cm into one nostril. Rotate the swabs against the inside of the nostril for 3 seconds. Apply slight pressure with a finger on the outside of the nose to ensure good contact between the swabs and inside of the nose. Using the same swabs, repeat for the other nostril. Place the swabs into the transport tube (swabs should stay attached to the red cap). Make sure the red cap is tightly on the transport tube. Transport: Room temperature up to 24 hours, otherwise refrigerate (2-8°C) up to 5 days.