Chromogenic Factor X
| Label Mnemonic: | CH10 |
| Epic code: | LAB1020 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 85260 |
Specimen(s):
Plasma More information
Collection Medium:
 |
| Light Blue top tube 2.7 mL (Na Citrate) |
Minimum:
Full draw; 2.7 mL light blue top
Rejection Criteria:
Short drawn tube, clot, traumatic tap (excessive hemolysis).
Testing
Schedule:
0800-1630 Monday, Wednesday and Friday. If you need results STAT, call Hematology resident (0800-1630, M-F) at 356-8628. All other times page the Pathology resident on-call at 3404.
Turn Around
Time:
24-36 hours
Reference Range:
59 – 130%
Therapeutic reference range is 20 – 40%
Therapeutic reference range is 20 – 40%
Interpretive Data:
An approximate Chromogenic Factor X range of 20-40% corresponds to a therapeutic INR range of 2.0-3.0. This assay can be used for monitoring warfarin therapy in patients with lupus anticoagulants. Anti-phospholipid antibodies may influence the prothrombin time and resulting INR calculation, causing it to not accurately reflect the true level of anticoagulation.
Rosborough, T.K. and Shepherd, M.F. Unreliability of International Normalized Ratio for Monitoring Warfarin Therapy in Patients with Lupus Anticoagulant. Pharmacotherapy 2004;24(7):838-842.
McGlasson, D.L., Romick, B.G., and Rubal, B.J. Comparison of a Chromogenic Factor X Assay with International Normalized Ratio for Monitoring Oral Anticoagulation Therapy. Blood Coagulation and Fibrinolysis 2008;19:513-517.
Rosborough, T.K. and Shepherd, M.F. Unreliability of International Normalized Ratio for Monitoring Warfarin Therapy in Patients with Lupus Anticoagulant. Pharmacotherapy 2004;24(7):838-842.
McGlasson, D.L., Romick, B.G., and Rubal, B.J. Comparison of a Chromogenic Factor X Assay with International Normalized Ratio for Monitoring Oral Anticoagulation Therapy. Blood Coagulation and Fibrinolysis 2008;19:513-517.
Test
Limitations:
This test is to be used for coumadin monitoring of patients with
positive Lupus anticoagulants.
Methodology:
Activity detection by chromogenic substrate
Sample
Processing:
Centrifuge within four hours of draw time.
Separate plasma into plastic container and cap (glass is not acceptable).
Centrifuge a second time to ensure platelet poor plasma and separate plasma into a new, labeled plastic tube, cap.
Separate plasma into plastic container and cap (glass is not acceptable).
Centrifuge a second time to ensure platelet poor plasma and separate plasma into a new, labeled plastic tube, cap.
Sample
Storage:
Freeze specimen immediately.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container.
CPT Code:
85260