N-terminal-pro-BNP
| Label Mnemonic: | PBNP |
| Epic code: | LAB649 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 83880 |
Specimen(s):
Plasma
Collection Medium:
|
| Plasma Separator Tube 4.5 mL |
Minimum:
3 mL plasma separator tube top or TWO Microtainer®
devices.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Age Reference Range (pg/mL)
Pediatric (boys and girls)
0-30 days 263 - 6500
1 month - 11 months 37 - 1000
12 months - 35 months 39 - 675
3 years to 6 years 23 - 327
7 years to 14 years 10 - 242
15 years to 18 years 6 - 207
Males
19-44 years 0 - 93
45-54 years 0 - 138
55-64 years 0 - 177
65-74 years 0 - 229
75 years or older 0 - 852
Females
19-44 years 0 - 178
45-54 years 0 - 192
55-64 years 0 - 226
65-74 years 0 - 353
75 years or older 0 - 624
Reference ranges in adults reflect 95th percentiles for NT-pro-BNP
levels in patients without congestive heart failure (CHF). Knowledge
of each individual patient's NT-proBNP range may be more useful than
using similar cut-points for every patient.
For adult chronic CHF patients according to New York Heart Association
(NYHA) Functional Class for NT-proBNP levels in pg/mL:
Mean 5th percentile 95th percentile
Class I: 1016 33 3410
Class II: 1666 103 6567
Class III: 3029 126 10449
Class IV: 3465 148 12188
Pediatric reference ranges for patients 18 years and younger are from
reference (1).
Among patients with dyspnea, NT-proBNP is highly sensitive for the
detection of acute CHF. In addition, a NT-proBNP < 300 pg/mL
effectively rules out acute CHF, with 99% negative predictive value.
Elevations in NT-proBNP levels may be observed in states other than
left ventricular congestive failure including: acute coronary
syndromes, right heart strain/failure (including pulmonary embolism
and cor pulmonale), critical illness, and renal failure. Falsely low
NT-proBNP in CHF patients may be observed in increased body mass index.
References:
(1) Nir A et al. Pediatr Cardiol 30:3-8, 2009.
Test Limitations:
Hemolysis H = 1000 Lipemia L = 1500 Icterus I = 25 Biotin <3500 ng/mL
Methodology:
Electrochemiluminescence Immunoassay
Assay updated with increased tolerance to biotin on 05/02/23
Assay updated with increased tolerance to biotin on 05/02/23
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays
NT-Pro-BNP is stable for three days at 2-8°C, so this assay may be added to existing PST tubes during those three days.