Tobramycin Drug Level
Label Mnemonic: TOB
Epic Lab Code: LAB36
Order Form: Laboratory Requisition
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
3 mL whole blood in light green top or ONE Microtainer®.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Peak 6-10 μg/mL; (45-75 min. after I.M. dose, 15-30 min. after I.V. dose) Trough <2 μg/mL; (Not more than 30 min. before next dose) Critical value: Peak >10 μg/mL
Phlebotomy team does not draw timed tobramycin specimens. Clinical staff must draw accurately timed peak and trough specimens.
Test Limitations:
Icterus: No significant interference from bilirubin up to an I index of 30. Hemolysis: No significant interference from hemoglobin up to an H index of 800. Lipemia (Intralipid): No significant interference up to an L index of 750. There is poor correlation between turbidity and triglycerides concentration. No significant interference from total protein up to 12 g/dL.
Homogenous Enzyme Immunoassay
Sample Processing:
Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.
Label pre- or post- specimens clearly.
Sample Storage:
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container.
CPT Code:
See Additional Information:
Chemistry Critical Lab Values