Tacrolimus

Label Mnemonic:	TACRO
Epic code:	LAB908
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	80197

Specimen(s):

Whole Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

Preferred Minimum: 3 mL whole blood in lavender top tube (EDTA) or ONE lavender top (EDTA) microtube for pediatric patients, mix well.

Testing Schedule:

Results are available 1200 Monday-Sunday. Samples need to be in lab by 0900 for results at 1200 (daily).

Turn Around Time:

24 hours (upon receipt in laboratory)

Interpretive Data:

Analysis performed using the Roche Diagnostics Elecsys Tacrolimus immunoassay on the Roche e602 platform. The general therapeutic range for tacrolimus is 5-20 ng/mL. The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center.

Comments:

Analysis performed using the Abbott Diagnostics Tacrolimus immunoassay on the Architect platform. The general therapeutic range for tacrolimus is 5-20 ng/mL. The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center. Tacrolimus whole blood concentrations can be measured by either immunoassay or chromatographic methodologies. These two methodologies are not directly interchangeable, and the measured tacrolimus whole blood concentration depends on the methodology used. Reference ranges may vary according to the specific immunoassay or HPLC-MS/MS test. Generally, immunoassays have been reported to have a positive bias relative to HPLC-MS/MS assays due to the detection of antibody cross- reactivity with tacrolimus metabolites.

Methodology:

Chemiluminescent Microparticle Immunoassay

Instructions:

Trough sample preferred.

Sample Processing:

Submit whole blood in original container. DO NOT CENTRIFUGE.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

80197