Protein Electrophoresis
Label Mnemonic: | SPE |
Epic code: | LAB953 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
Specimen(s):
Serum
Collection Medium:
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Red top tube 5 mL (Clot Activator) |
Minimum:
2 mL whole blood or one microtube for pediatric patients includes total protein.
Testing Schedule:
Daily - Monday-Friday
Turn Around Time:
48 Hours
Reference Range:
Albumin 3.7 - 5.2 g/dl Alpha1 0.3 - 0.6 g/dl Alpha2 0.3 - 0.8 g/dl Beta 0.5 - 1.1 g/dl Gamma 0.6 - 1.5 g/dl
Methodology:
Capillary Electrophoresis
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells.
Separate plasma or serum into labeled container and cap.
Separate plasma or serum into labeled container and cap.
Sample Storage:
Refrigerate.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Capillary electrophoresis can resolve separate beta-1 and beta-2 fractions, although a reference range is only available for the total beta fraction.
Reference ranges changed on 10/17/2023. The reference intervals published by Katzmann et al. were verified as appropriate for the UIHC population.
Katzmann JA, et al. Prospective study of serum protein capillary zone electrophoresis and immunotyping of monoclonal proteins by immunosubtraction. Am J Clin Pathol. 1998 Oct;110(4):503-9. doi: 10.1093/ajcp/110.4.503. PMID: 9763037.
The table below shows the proteins that predominantly make up the fractions of electrophoresis: