Plasma

Plasma Separator Tube 4.5 mL

Call laboratory for additional acceptable specimen collection containers.

3 mL whole blood in light green top tube or TWO Microtainer^® devices.

24 hrs/day, 7 days a week, including holidays.

1 hour (upon receipt in laboratory)

Females:
  Adult females:
    Follicular phase:      0.2 - 1.5 ng/mL
    Ovulation phase:       0.8 - 3.0 ng/mL
    Luteal phase:          1.7 - 27.0 ng/mL
    Post-menopausal:       < 0.2 - 0.8 ng/mL
  Pediatric females:
    < 2 years old:         0.87 - 3.37 ng/mL
    2-9 years old:         0.20 - 0.24 ng/mL
    10-17 years old:       adult levels generally achieved by puberty

Males:
  Adult males:             0.2 - 1.4 ng/mL
    < 2 years old          0.87 - 3.37 ng/mL
    2-9 years old          < 0.2 ng/mL
    10-17 years old        adult levels generally achieved by puberty

Reference: Lippe BM, LaFranchi SH, Lavin N, et al: Serum 17-alpha-
hydroxyprogesterone, progesterone, estradiol, and testosterone in the 
diagnosis and management of congenital adrenal hyperplasia. J Pediatr 
1974;85:782-787.

This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely increased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

The assay is unaffected by icterus = 54(bilirubin ditaurate is 
less than 54 mg/dL), hemolysis = 1000 (Hb is less than 1.0 g/dL), 
lipemia = 200 (Intralipid is less than 720 mg/dL) and biotin is less 
than 20 ng/mL. (criterion: recovery within plus or minus 10% of 
initial value).

No interference was observed from rheumatoid factors up to a 
concentration of 2000 IU/mL.

In vitro tests were performed on 18 commonly used pharmaceuticals. Of 
these, only phenylbutazone at therapeutic dosage levels showed 
interference with the assay (progesterone values depressed).

As with all tests containing monoclonal antibodies, erroneous findings 
may be obtained from samples taken from patients who have been treated 
with monoclonal mouse antibodies or who have received them for 
diagnostic purposes.

In rare cases, interference due to extremely high titers of antibodies 
to ruthenium can occur.

Progesterone II contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the Progesterone II findings should always be 
assessed in conjunction with the patient's medical history, clinical 
examination and other findings.

Electrochemiluminescence Immunoassay

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

See Additional Information:
Biotin Interference with Immunoassays