HCG - Pregnancy, Serum, Quantitative
Label Mnemonic: SPGPOC
Epic code: LAB1148
Order form: Laboratory Requisition
Collection Medium:
Plasma Separator Tube 4.5 mL
3 mL whole blood in light green top tube or TWO Microtainer® devices.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
30 minutes (upon receipt in laboratory)
Reference Range:
Weeks of gestation      Expected range
3 weeks                     5 - 72
4 weeks                    10 - 708
5 weeks                   217 - 8,245
6 weeks                   152 - 32,177
7 weeks                 4,059 - 153,767
8 weeks                31,366 - 149,094
9 weeks                59,109 - 135,901
10 weeks               44,186 - 170,409
12 weeks               27,107 - 201,165
14 weeks               24,302 - 93,646
15 weeks               12,540 - 69,747
16 weeks                8,904 - 55,332
17 weeks                8,240 - 51,793
18 weeks                9,649 - 55,271

Non-pregnant females:   < 3 mIU/mL
Males:                  < 2 mIU/mL

Healthy non-pregnant peri-menopausal and post-menopausal females may 
have HCG values up to 8 mIU/mL.  Heterophile antibodies present in the 
serum of some patients may cause a false positive result in this 
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

This assay recognizes the intact HCG "holo-hormone" produced in pregnancy but may not recognize other forms of HCG (e.g., "nicked HCG") produced in other conditions such as tumors of the germs cells, ovaries, bladder, pancreas, stomach, lungs, and liver.
Electrochemiluminescence Immunoassay
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample Storage:
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
See Additional Information:
Biotin Interference with Immunoassays