Plasma

Plasma Separator Tube 4.5 mL

Call laboratory for additional acceptable specimen collection containers.

3 mL whole blood in light green top tube or ONE Microtainer^® for pediatric patients.

24 hrs/day, 7 days a week, including holidays.

1 hour (upon receipt in laboratory)

 

        AGE                   MALES         FEMALES
       0 - 14 days         83-248 U/L     83-248 U/L
 15 days - 12 months      122-469 U/L    122-469 U/L
       1 - 9 years        142-335 U/L    142-335 U/L
      10 - 12 years       129-417 U/L    129-417 U/L
      13 - 14 years       116-468 U/L     57-254 U/L
      15 - 16 years        82-331 U/L     50-117 U/L
      17 - 18 years        55-149 U/L     45-87 U/L
19+ years [unchanged]      40-129 U/L     35-104 U/L

Pediatric reference ranges updated 5/23/2017 (see reference below).

Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I Index of 60 for 
conjugated bilirubin corresponding to approximately 60 mg/dL 
conjugated and unconjugated bilirubin.

Hemolysis: No significant interference up to an H index of 500 
(approximate hemoglobin concentration: 500 mg/dL).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Reference
Estey MP, Cohen AH, Colantonio DA, et al. CLSI-based transference of 
the CALIPER database of pediatric reference intervals from Abbott to 
Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: Direct 
validation using reference samples from the CALIPER cohort. Clin 
Biochem 2013;46:1197-1219.

Colorimetric

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.