Lupus Anticoagulant
| Label Mnemonic: | LUPUS |
| Epic code: | LAB478 |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | Dilute Viper Venom Test - 85613 Clinical Pathology Consultation Limited - 80500 Prothrombin Time - 85610 Thromboplastin Time Partial Plasma/Whole Blood - 85730 Fibrinogen Activity - 85384 Thrombin Time Plasma - 85670 Thromboplastin Time Prtl Substit Plasma Frctj Ea - 85732 Heparin Assay - 85520 Hexagonal LA - 85598 Hepzyme Heparin Neutralization - 85525 |
Specimen(s):
Citrated Whole Blood More information
Collection Medium:
 | and | |
| Light Blue top tube 2.7 mL (Na Citrate) | Light Blue top tube 2.7 mL (Na Citrate) |
Minimum:
Collect TWO full tubes - 2.7 mL citrated (light blue top) tube
Rejection Criteria:
Short drawn tube, clot, traumatic tap (excessive hemolysis). Add on testing is not allowed.
Testing
Schedule:
0800-1630 Monday, Wednesday and Friday. If you need results STAT, call Hematology resident (0800-1630, M-F) at 356-8628. All other times page the Pathology resident on-call at 3404.
Turn Around
Time:
24-36 hours
Methodology:
A prolonged PTT is investigated to determine the presence of a Lupus
Anticoagulant.
Sample
Processing:
Double-centrifuge specimen to prepare platelet-free plasma.
Immediately centrifuge specimen at 3500 RPM for 12 minutes.
Using a plastic transfer pipette, carefully remove plasma from cells avoiding the platelet/buffy coat.
Place plasma into a labeled plastic tube.
Centrifuge plasma again at 3500 RPM for 12 minutes.
Remove the top of the plasma and place into a labeled plastic tube.
Freeze double-spun plasma within 4 hours of collection time at -20 degrees C or below.
Immediately centrifuge specimen at 3500 RPM for 12 minutes.
Using a plastic transfer pipette, carefully remove plasma from cells avoiding the platelet/buffy coat.
Place plasma into a labeled plastic tube.
Centrifuge plasma again at 3500 RPM for 12 minutes.
Remove the top of the plasma and place into a labeled plastic tube.
Freeze double-spun plasma within 4 hours of collection time at -20 degrees C or below.
Sample
Storage:
Freeze specimen immediately.
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Samples received thawed will be cancelled.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
Samples received thawed will be cancelled.