Chronic Lymphocytic Leukemia
Order form: Comprehensive Hematopathology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 88184 (first antibody), 88185(for each additional antibody) technical; 88189 professional; T cell or NK cell disorder: 88184, 88185(x15) technical; 88188 professional
Specimen(s):
Various
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)
Minimum:
Peripheral Blood: 8.5 mL

Bone Marrow:  2-4 mL yellow top tube (ACD solution A)

Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue 
culture media.
Testing Schedule:
0800-1630 Monday through Friday.
Turn Around Time:
2 days
Reference Range:
Antibodies routinely included are: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD14, CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD56, CD103, CD138, CD200, FMC7, Kappa and Lambda. Antibodies will also identify cases of rolymphocytic and Hairy Cell Leukemia.

If clinical or morphologic features suggest a "T" or "NK" lymphocyte disorder, then additional antibody combinations may be performed.

The pathologist will provide an interpretative report.
Comments:
LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

For further details, refer to How to Send Flow Cytometry Specimens.

Please state the clinical question to be answered on the requisition. Maintain the specimen at room temperature; do not refrigerate or incubate.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Comprehensive Hematopathology Requisition before shipping the specimen.
Methodology:
Flow Cytometry-Lysed Whole
Sample Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Sample Storage:
Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.
CPT Code:
88184 (first antibody), 88185(for each additional antibody) technical; 88189 professional; T cell or NK cell disorder: 88184, 88185(x15) technical; 88188 professional