Lactate Dehydrogenase (LDH)
Label Mnemonic: | LD |
Epic code: | LAB96 |
Order form: | Laboratory Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: | 83615 |
Specimen(s):
Plasma
Collection Medium:
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Plasma Separator Tube 4.5 mL |
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection
containers.
Minimum:
3 mL whole blood in light green top tube or ONE Microtainer®
for pediatric patients.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Adult Males 135-225 U/L Adult Females 135-214 U/L Pediatric Reference Ranges Male Female 0-31 days 125-735 U/L 145-765 U/L 31 days-1 year 170-450 190-420 1 year-4 years 155-345 165-395 4 years-7 years 155-345 135-345 7 years-10 years 145-300 140-280 10 years-13 years 120-290 120-260 13 years-16 years 120-290 100-275 16 years-19 years 105-235 105-230
Comments:
Refer to BD Microtainer® Tubes
product sheet
for detailed sample collection instructions.
Avoid hemolysis. False elevations may occur in specimens which are not processed promptly to separate serum from RBC's.
Avoid hemolysis. False elevations may occur in specimens which are not processed promptly to separate serum from RBC's.
Test Limitations:
Criterion: recovery plus or minus 10% of initial values Plasma Icterus: No significant interference up to an I index of 60 (approximate conjugated and unconjugated bilirubin concentration: 60 mg/dL). Hemolysis: RBC contamination elevates results; interference if H index is greater than or equal to 50. Lipemia (Intralipid): No significant interference up to an L index of 900. There is poor correlation between turbidity and triglycerides concentration. Highly lipemic samples may cause ABS! flags.
Methodology:
UV Assay
Sample Processing:
Centrifuge at a speed and time necessary to get barrier separation
of plasma/serum and cells within 1 hour of collection. Send specimen
in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal
bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
See also:
Lactate Dehydrogenase (LDH)-Other, Body Fluid
Lactate Dehydrogenase (LDH)-Other, Body Fluid