Lymphocyte Subsets Flow Cytometry Panel

Label Mnemonic: TBNK
Epic code: LAB9383
Order form: Comprehensive Hematopathology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 88184, 88185 x5 Technical, 88187 Professional
Specimen(s):
Peripheral Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Alternate Collection Media:
Pink top tube 6 mL (K2-EDTA)
Minimum:

Adult: 3 mL whole blood in a purple top tube (EDTA)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

Testing Schedule:

Samples are batched and will be processed during the hours of 0800–1600 daily. Sample stability is 24 hours, samples older than 24 hours will be cancelled as unacceptable. Samples that arrive on weekends or holidays will be triaged and processed during the next business day. For additional services, contact Clinical Pathology Resident on-call at pager #3404

Turn Around Time:
24 hours (upon receipt in laboratory)
Reference Range:
The pathologist will provide an interpretative report.  

Adult reference ranges for peripheral blood by whole blood lysis 
method using flow cytometry:

B cells (CD19)                      6-22%
T cells (CD3)                       65-85%
T helper/induce (CD4+/CD3+)         30-62%
T cytotoxic/suppressor (CD8+/CD3+)  13-37%
NK cells (CD16+/CD56+/CD3-)         5-31%
CD3+/CD16+/CD56+                    0-6%
CD4/CD8 ratio                       0.7-2.7

Age specific pediatric reference ranges will be provided with the 
interpretive report.
Comments:


For further details, refer to How to Send Flow Cytometry Specimens.

Methodology:

Flow Cytometry-Whole Blood Lysis

Sample Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.
Sample Storage:
Room Temperature.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.