Immunoglobulin G (IgG), Individual Quant

Label Mnemonic:	IGG
Epic code:	LAB71
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	82784

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or ONE Microtainer^®.

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

700-1600 mg/dL

Children and Juveniles
 0-1 year    232-1411 mg/dL
 1-3 years   453-916  mg/dL
 4-6 years   504-1465 mg/dL
 7-9 years   572-1474 mg/dL
10-11 years  698-1560 mg/dL
12-13 years  759-1550 mg/dL
14-15 years  716-1711 mg/dL
16-19 years  549-1584 mg/dL

Soldin JS, Brugnara C, Wong EC. Pediatric Reference Intervals. AACC Press. 2005, 5th ed., p. 42.

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dL).

Hemolysis: No significant interference up to an H index of 1000. 
(approximate hemoglobin concentration: 1000 mg/dL)

Lipemia (Intralipid): No significant interference up to an L index of 
2000.

There is poor correlation between turbidity and triglycerides 
concentration.

There is no cross-reaction between IgG, IgA and IgM under the assay 
conditions.

Sera with ill-defined clinical characteristics should first be 
subjected to protein electrophoresis so that an antigen excess (e.g. 
gammopathy), if present, can be identified.

IgG concentrations of more than 40,000 mg/dL (400 g/L) can cause a 
high-dose hook effect.

Methodology:

Immunoturbidimetric

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82784