Immunoglobulin A (IgA), Individual Quant

Label Mnemonic:	IGA
Epic code:	LAB73
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	82784

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or ONE Microtainer^®

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

70-400 mg/dL

Children and Juveniles
 0-1 year      0-83 mg/dL
 1-3 years   20-100 mg/dL
 4-6 years   27-195 mg/dL
 7-9 years   34-305 mg/dL
10-11 years  53-204 mg/dL
12-13 years  58-358 mg/dL
14-15 years  47-249 mg/dL
16-19 years  61-348 mg/dL
20-adult     70-400 mg/dL

Comments:

Refer to BD Microtainer^® Tubes product sheet for detailed sample collection instructions.

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial value.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dL).

Hemolysis: No significant interference up to an H index of 1000 
(approximate hemoglobin concentration: 1000 mg/dL).

Lipemia (Intralipid): No significant interference up to an L index of 
2000). There is poor correlation between turbidity and triglycerides 
concentration.

There is no cross-reaction between IgA and IgG or IgM under the assay 
conditions.

Plasma with ill-defined characteristics should be subject to protein 
electrophoresis to identify a possible antigen excess (e.g. 
gammopathy).

A high-dose hook effect may occur at concentrations above 12,000 mg/dL 
(120 g/l).

Methodology:

Immunoturbidimetric

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

82784