Plasma

Call laboratory for additional acceptable specimen collection containers.

3 mL whole blood from light green top tube or TWO Microtainer^® devices for pediatric patients

24 hrs/day, 7 days a week, including holidays.

1 hour (upon receipt in laboratory)

Non-reactive; positive with vaccine administration. Non-reactive samples have anti-HBs level less than 8.5 mIU/mL. Reactive samples have anti-HBs level greater than or equal to 11.5 mIU/mL. The result is indeterminate if the sample tests twice with anti-HBs results greater than or equal to 8.5 mIU/mL but less than 11.5 mIU/mL.

This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

Part of initial diagnostic hepatitis profile or to verify immunity status in patients who have received the hepatitis B vaccine. Results include reactivity (REACTIVE or NON-REAC), immune status (IMMUNE or NON IMM OR INDETERM) and quantitative concentration. Anti-HBs can be formed following a hepatitis B infection or after hepatitis B vaccination.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).

Assay is unaffected by icterus (<60 mg/dL), hemolysis (Hb < 1600 mg/dL), and lipemia (triglycerides < 1500 mg/dL). No false negative findings due to the high-dose hook effect are observed up to an anti-HBs concentration of 115,000 mIU/mL. In rare cases, interference due to extremely high titers of antibodies to analyte- specific antibodies, streptavidin, or ruthenium can occur.

For diagnostic purposes, the results should always be assessed in conjunction with the patient's medical history, clinical examination, and other findings. The assay is not intended for use in screening blood bank donors. A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B. Individuals who have received blood component therapy, e.g., whole blood, plasma, immune globulin administration, during the previous 3 to 6 months may have a false reactive anti-HBs due to passive transfer of anti-HBs. Results from immunosuppressed individuals should be interpreted with caution.

Electrochemiluminescence Immunoassay (ECLIA)

86706

See Additional Information:
Biotin Interference with Immunoassays