Hepatitis Be Antigen
Label Mnemonic: HBEAG
Epic Lab Code: LAB3297
Downtime Form: A-1a General Laboratory Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Whole Blood
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO Microtainer® devices for pediatric patients
Rejection Criteria:
Heat-inactivated, grossly hemolyzed, or lipemic specimens. Specimens containing particulate material, or collected in citrate-based anticoagulant.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
See Interpretive Data
Interpretive Data:
A negative result of <0.90 indicates that Hepatitis Be antigens were not detected in the sample. A negative test result does not exclude the possibility of exposure to or infection with HBV.

A positive result of >1.10 indicates presumptive evidence of hepatitis B virus infection. Hepatitis Be antigens were detected in the sample which suggests either on-going or previous HBV infection.
Comments:
Order this test only when specimen is repeatedly reactive for Hepatitis B Surface Antigen.

Assay changed to Roche Elecsys HBeAg on 10/03/2017.
Test Limitations:
Assay is unaffected by icterus (<25 mg/dL), hemolysis (Hb <1600 mg/dL), lipemia (triglycerides <1500 mg/dL) and total protein < 12 g/dL. In patients receiving high biotin doses (> 5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration.

For diagnostic purposes, the results should always be assessed in conjunction with the patient's medical history, clinical examination, and other findings. A reactive HBeAg result does not exclude co- infection by another hepatitis virus. False positive results due to non-specific reactivity cannot be ruled out with this assay. In rare cases, interference due to extremely high titers of antibodies to immunological components, streptavidin and ruthenium can occur. A non- reactive test result does not exclude the possibility of exposure to HBV. Negative HBeAg results may occur during early infection due to delayed seroconversion. False negative results may occur due to antigen levels below the detection limit of this assay or if the patient's antigen does not react with the antibody used in this test.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
87350