Hepatitis B Core Antibody, Total
Label Mnemonic: HBCB
Epic Lab Code: LAB622
Downtime Form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
5 mL whole blood from light green top tube or TWO light green top microtubes for pediatric patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Interpretive Data:
A negative result indicates that anti-HBc antibodies were not detected in the sample. A positive result indicates presumptive evidence of hepatitis B virus infection. Anti-HBc antibodies were detected in the sample which suggests either on-going or previous HBV infection.
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely increased (i.e., falsely negative) results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

Part of initial diagnostic hepatitis profile.

Current methods for the detection of anti-HBc antibodies may not detect all infected individuals. A negative test result does not exclude the possibility of exposure to or infection with HBV.
Test Limitations:
The assay is unaffected by icterus < 25 mg/dL, hemolysis (Hb < 800 mg/dL), or lipemia < 1000 mg/dL).
Electrochemiluminescence Immunoassay

Updated to Roche Elecsys Anti-HBc II assay on 11/9/18
CPT Code:
See Additional Information:
Biotin Interference with Immunoassays