Hepatitis B Core Antibody, IgM
Label Mnemonic: HBCM
Epic Lab Code: LAB624
Downtime Form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO Microtainer® devices for pediatric patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
See Interpretive Data
Interpretive Data:
A negative result of <0.90 indicates that IgM anti-HBc antibodies were not detected in the sample. A negative test result does not exclude the possibility of exposure to or infection with HBV.

A positive result of >1.10 indicates presumptive evidence of hepatitis B virus infection. IgM anti-HBc antibodies were detected in the sample which suggests either on-going or previous HBV infection.
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.

Current methods for the detection of IgM anti-HBc may not detect all infected individuals. False negative results may occur due to antibody levels below the detection limit of this assay or if the patient's antibodies do not react with the antigen used in this test. A positive anti-HBc IgM result does not exclude co-infection by another hepatitis virus.

The assay is limited to the detection of IgM anti-HBc in human serum or plasma. It can be used to determine whether a patient has, or has recently had, acute or subclinical hepatitis B infection. Supportive clinical information, including other hepatitis B markers, should also be evaluated. The test cannot determine a patient's immune status to hepatitis B.
Test Limitations:
The assay is unaffected by icterus < 25 mg/dL, hemolysis (Hb < 2000 mg/dL, or lipemia < 1000 mg/dL.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
86705
 
See Additional Information:
Biotin Interference with Immunoassays