Hemoglobin A1C
Label Mnemonic: A1C
Epic Lab Code: LAB90
Downtime Form: A-1a General Laboratory Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL; lavender top or TWO full lavender Microtainer® devices for pediatrics
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
4.8 - 6.0%
Glycemic control guidelines:
  Non-diabetic <6%
  Goal <7%
  Therapeutic action >8%
Comments:
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Test Limitations:
Rheumatoid factor less than 750 IU/mL and ascorbic acid less than 50 mg/dL do not interfere. In hemolytic anemia and iron deficiency anemia, the average age of erythrocytes is altered. Caution should be used when interpreting the HbA1c results from patients with these conditions (2, 3). This assay is not useful in judging day-to-day glucose control and should not be used to replace daily home testing of urine and blood glucose. References: (1) Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474. (2) Greiling H, Gressner AM, eds. Lehrbuch der Klinischen Chemie und Pathobiochemie, 3rd ed. Stuttgart/New York: Schattauer, 1995:279. (3) Bessis M. Living Blood Cells and their Ultrastructure. Berlin: Springer-Verlag; 1973.
Methodology:
Turbidimetric Immuno inhibition
CPT Code:
83036