Alpha Fetoprotein
Label Mnemonic: AFP
Epic code: LAB554
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood in light green top tube or TWO Microtainer® devices for pediatric patients.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
                             All units in ng/mL
Age                     Male                   Female
------                  -------------          ------------
0-13 days               5,000 - 105,000        5,000 - 105,000
14-30 days              300 - 60,000           300 - 60,000
1 month                 100 - 10,000           100 - 10,000
2 months                40 - 1,000             40 - 1,000
3 months                11 - 300               11 - 300
4 months                5 - 200                5 - 200
5 months                0 - 90                 0 - 90
6-11 months             0 - 60                 0 - 97
1 year                  0 - 17                 0 - 41
2 years                 0 - 12                 0 - 12
3+ years                0 - 9                  0 - 9

Reference ranges updated 6/30/2011 by addition of pediatric reference 
ranges below 3 years old.
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.
Test Limitations:
Hemolysis: No significant interference up to an H index of 2200 (approximate hemoglobin concentration: 2200 mg/dL).

Lipemia: No significant interference up to an L index of 1500 (approximate triglycerides concentration: 3000 mg/dL). Note there is poor correlation between turbidity and triglyceride concentration.

Icterus: No significant interference up to an I index of 65 (approximate conjugated and unconjugated bilirubin concentration: 65 mg/dL).
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
82105
 
See Additional Information:
Biotin Interference with Immunoassays