Glucose-6-Phosphate Dehydrogenase

Label Mnemonic: G6PDA
Epic code: LAB3221
Downtime form: Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Commercial Mailout Laboratory
6240-8 RCP
356-8593
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Alternate Collection Media:
Pink top tube 6 mL (K2-EDTA) or Green top tube 4 mL (Na Heparin)
Minimum:

Preferred Minimum: 3 mL whole blood
Absolute Minimum: 1.5 mL whole blood

Rejection Criteria:

Clotted or hemolyzed specimens.

Turn Around Time:

1-3 days upon receipt at reference laboratory

Reference Range:
Age Reference Intervals (U/g Hb)
Less than 8 days 16.4 - 24.1
8 - 30 days 13.6 - 23.9
1 - 6 months 11.5 - 21.5
7 - 12 months 10.3 - 17.9
1 - 17 years 9.7 - 16.9
Greater than or equal to 18 years 9.6 - 16.3

 

Interpretive Data:

World Health Organization guidance on classification of G6PD activity is as follows:

 

Males: G6PD activity less than 30% of the normal median are regarded as G6PD deficient. Males with G6PD activity of 30% or more of the normal median can be regarded as G6PD normal.

 

Females: G6PD activity less than 30% of the normal median are regarded as G6PD deficient. Females with G6PD activity of 80% or more of the normal median can be regarded as G6PD normal. G6PD activity between 30% and 80% of the normal median are regarded as intermediate activity.

 

Cutoffs and results are specific to this G6PD assay and configuration and cannot be used interchangeably across different assays, parameters, and/or instrument configurations.

 

Reference: Guide to G6PD deficiency rapid diagnostic testing to support P. vivax radical cure. Geneva: World Health Organization; 2018. ISBN 978-92-4-151428-6

 

Percent of Normal Activity (U/g Hb)
Age 100% 80% 30%
<8 days 19.7 15.8 5.9
8 - 30 days 18.2 14.6 5.5
1 - 6 months 16.1 12.9 4.8
7 - 12 months 13.8 11.0 4.1
1 - 17 years 12.9 10.3 3.9
18 years 12.7 10.2 3.8
Percentage of normal activity cutoffs to G6PD enzyme activity. 100% for males and females is defined as the 50th percentile of non-affected males.
Comments:

Note: Patients who have recently received transfusions have normal donor cells that may mask G-6-PD deficient erythrocytes.

Methodology:

Quantitative Enzymatic

CPT Code:
82955