Follicle Stimulating Hormone (FSH)
Label Mnemonic: FSH
Epic Lab Code: LAB86
Downtime Form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
3 mL in light green top tube or TWO Microtainer® devices
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
FEMALES - menstruating: Follicular phase 3.5 - 12.5 mIU/mL Ovulation phase 4.7 - 21.5 mIU/mL Luteal phase 1.7 - 7.7 mIU/mL Postmenopause 25.8 - 134.8 mIU/mL MALES: 1.5 - 12.4 mIU/mL AGE MALE FEMALE 1 - 3 years 0.2-1.5 mIU/mL 1.2-5.7 mIU/mL 4 - 8 years 0.5-1.6 mIU/mL 0.8-3.0 mIU/mL Tanner I 0.7-3.1 mIU/mL 0.5-5.1 mIU/mL Tanner II 1.1-6.9 mIU/mL 2.4-8.7 mIU/mL Tanner III 1.8-6.2 mIU/mL 3.8-8.1 mIU/mL Tanner IV 1.8-4.8 mIU/mL 1.1-9.6 mIU/mL Tanner V 1.4-6.8 mIU/mL 2.0-7.6 mIU/mL
This assay may be significantly impacted by high-dose biotin (>5 mg dose) taken within previous 12 hours. High concentrations of biotin may lead to falsely decreased results. These concentrations may be found in patients taking over-the-counter supplements with biotin content much higher than nutritional requirements for biotin. Specimens should not be collected until at least 12 hours after the last dose.
Test Limitations:
The assay is unaffected by icterus (bilirubin is less than 64 mg/dL), hemolysis (Hb is less than 1,000 mg/dL), lipemia (Intralipid is less than 1900 mg/dL) and biotin is less than 60 ng/mL. (criterion: recovery within plus or minus 10% of initial value). No interference was observed from rheumatoid factors up to a concentration of 2250 U/mL. No high dose hook effect at FSH concentrations of up to 2000 mIU/mL. In vitro tests were performed on 17 commonly used pharmaceuticals. No interference with the assay was found. As with all tests containing monoclonal antibodies, erroneous findings may be obtained from samples taken from patients who have been treated with monoclonal mouse antibodies or who have received them for diagnostic purposes. In rare cases, interference due to extremely high titers of antibodies to ruthenium can occur. FSH contains additives which minimize these effects. Extremely high titers of antibodies to streptavidin can occur in isolated cases and cause interference. For diagnostic purposes, the FSH findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Electrochemiluminescence Immunoassay
CPT Code:
See Additional Information:
Biotin Interference with Immunoassays