Leiden Variant Factor 5 & F2 20210G>A Variant Factor 2 with Interpretation
Label Mnemonic: PROTPCR
Epic Lab Code: LAB346
Downtime Form: A-1a Miscellaneous Request
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Adult minimum: 3 mL whole blood in lavender top tube (EDTA) Children minimum: 2 mL whole blood in lavender top tube (EDTA) Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing. Testing requires a dedicated collection tube.
Delivery Instructions:
Room temperature up to 24 hours. Refrigerate overnight, weekends and holidays.
Testing Schedule:
Weekly
Turn Around Time:
14 days
Reference Range:
Normal
Comments:
Genomic DNA is PCR amplified and the presence of the Factor 5 Leiden and Factor 2 mutations associated with venous thrombosis are assessed simultaneously by gel electrophoresis.
Methodology:
Polymerase Chain Reaction (PCR) - Amplification Refractory Mutation Systems (ARMS)
CPT Code:
81241 - Leiden Variant Factor 5
81240 - F2 20210G>A Variant Factor 2