Estradiol (E2)
Label Mnemonic: EDIOL
Epic Lab Code: LAB523
Downtime Form: A-1a General Laboratory Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO Microtainer® devices
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
Males (18+ years) 11 - 43 pg/mL Females, non-pregnant Follicular Phase 12 - 233 pg/mL Ovulation Phase 41 - 398 pg/mL Luteal Phase 22 - 341 pg/mL Postmenopause <5 - 138 pg/mL Pregnancy 1st Trimester 154 - 3,243 pg/mL 2nd Trimester 1,561 - 21,280 pg/mL 3rd Trimester 8,525 - >30,000 pg/mL Children (1-10 years) Boys <5 - 20 pg/mL Girls 6 - 27 pg/mL
Comments:
Assay methodology changed to Roche Diagnostics Estradiol III assay on April 20, 2015. Adult reference ranges updated as part of change in methodology. Adult male reference range further updated on March 7, 2017.
Test Limitations:
The assay is unaffected by icterus (bilirubin is less than 66 mg/dL), hemolysis (Hb is less than 1000 mg/dL), lipemia (Intralipid is less than 1000 mg/dL) and biotin is less than 36 ng/mL. (criterion: recovery within plus or minus 10% of initial value). In patients receiving therapy with high biotin doses (i.e. is greater than 5 mg/day) no sample should be taken until at least 8 hours after the last biotin administration. No interference was observed from rheumatoid factors up to a concentration of 1200 U/mL.

The estradiol assay shows significant cross-reactivity with fulvestrant, a medication used to treat breast cancer. Contact commercial mailouts (319-356-8593) if estradiol measurement is needed in a patient currently receiving fulvestrant. In rare cases interference due to extremely high titers of antibodies to streptavidin and ruthenium can occur. Estradiol III contains additives which minimize these effects. For diagnostic purposes, the Estradiol III findings should always be assessed in conjunction with the patient's medical history, clinical examination and other findings.
Methodology:
Electrochemiluminescence Immunoassay
CPT Code:
82670