Nicotine & Metabolite
Label Mnemonic: NICOU
Epic Lab Code: LAB414
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Random Urine
Collection Medium:
Yellow top round bottom tube (no additive)
Minimum:
Preferred Minimum: 4 mL random urine with no additives or preservatives
Absolute Minimum: 1 mL random urine with no additives or preservatives
Rejection Criteria:
Specimens exposed to repeated freeze/thaw cycles.
Turn Around Time:
1-4 days upon receipt at reference laboratory
Interpretive Data:
Drug and analytes covered: nicotine, cotinine (metabolite), 3-OH- cotinine (metabolite), nornicotine (metabolite),anabasine (tobacco biomarker). All drug and metabolites are the non-glucuronidated (free) form.

Positive cutoff:
Nicotine - 2 ng/mL
Cotinine - 5 ng/mL
3-OH-Cotinine - 50 ng/mL
Nornicotine - 2 ng/mL
Anabasine - 3 ng/mL

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

For medical purposes only; not valid for forensic use.
Comments:
Random urine collections acceptable.
Methodology:
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
CPT Code:
80323