Direct Antiglobulin Test
Label Mnemonic: DAT
Epic code: LAB5548
Downtime form: A-1a Doctor-Provider Orders - Pathology Blood Center
DeGowin Blood Center - Blood Bank
C271 GH
Collection Medium:
Pink top tube 6 mL (K2-EDTA) Lavender top tube 3 mL (EDTA)
Adults - 2 mL
Pediatrics - 1 mL or EDTA Microtainer®
Rejection Criteria:
Specimen must be labeled with patient's first and last name and medical record number. Specimens will be rejected if information is not on the label when received.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
2 hours (upon receipt in laboratory)
Reference Range:
Negative result means that no antibodies were detected on the patient's red cells using polyspecific antiglobulin or anti IgG, depending on the patient age. If the patient is greater than four months old, poly specific antiglobulin is used. If the patient is less than four months old, only IgG is used.
Monospecific testing for IgG and C3 complement is automatically performed when the polyspecific test is positive.

Elution performed per pathologist recommendation or clinician order.

Only monospecific testing of IgG will be performed on cord samples when mothers are alloimmunized, when mother's antibody status is unknown, or on samples from patients < 4 months old.
Tube test
CPT Code: