Fluorescence In-Situ Hybridization (FISH-Tumors)

Downtime form:

Shivanand R. Patil Cytogenetics & Molecular Laboratory
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)

Specimen(s):

Tumor Tissue

Minimum:

Specimen obtained aseptically according to your protocol. Collect specimen using sterile technique. DO NOT FREEZE OR CENTRIFUGE THE SPECIMEN. This analysis can be done on interphase nuclei or on metaphase cells. Contact the Cytogenetics Lab with questions.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection. After hours specimens should be taken to Specimen Control. For questions after hours, call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Preliminary results will be reported within 2-3 days and final results within 1-2 weeks.

Reference Range:

Male:  X and Y probe signals          Female:  two X probe signals
For autosomes, the reference range varies for each probe (set).  
Suitable for interphase and metaphase analysis.  Call lab for 
information.

Comments:

Epic code: CYT54

This test can be used for screening of sex chromosomes, for monosomies (eg., 5, 7, etc.), for trisomies (eg., +4, +8, +11, +12, +13, +14, +18, +19, +21, +22, etc.) or for detection of the gene fusion resulting from translocations in solid tumors.

If the desired FISH probe is not listed, call the laboratory.

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website

Methodology:

Fluorescence In-Situ Hybridization (FISH)

CPT Code:

88271 x # of probes
88275 x # of probes, 88291

See also:
Chromosomal Analysis, Bone Marrow (for acquired)

See Additional Information:
Cytogenetics and Molecular Testing