CBC with Automated Differential
Label Mnemonic: CBC, DIFF
Epic code: LAB293
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
6240 RCP
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Full draw; 3.0 mL lavender top; (or fingerstick-purple top microtainer®)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
  Adults Children (5-18 yrs)
  (mm3) (mm3)
Band Neutrophils - -
Neutrophils 2188-7800 1700-7500
Eosinophils 40-390 40-650
Basophils 10-136 7-140
Monocytes 130-860 28-825
Lymphocytes 875-3300 1250-7000
Immature Granulocytes - -
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.

A CBC must be ordered to perform a differential. Hematology instruments are automated differential screening instruments that identify WBC's by their size and staining activity. It performs a complete cell identification that includes neutrophils, lymphocytes, monocytes, basophils, eosinophils and immature granulocytes. The automated instruments count 32,000 white blood cells and statistically provide a more accurate differential count than a manual differential. All differentials will be analyzed on the instrument first. If the results fail the screening criteria, a manual differential will be performed. Pathologist approval is required to perform a manual differential on a specimen with an automated differential that passed the screening criteria.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain
CPT Code:
See Additional Information:
Hematology Pediatric Reference Ranges