CBC with Automated Differential
Label Mnemonic: CBC, DIFF
Epic Lab Code: LAB293
Downtime Form: A-1a General Laboratory Requisition
Hematology
6240 RCP
356-3527
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
Full draw; 3.0 mL lavender top; (or fingerstick-purple top microtainer®)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
  Adults Children (5-18 yrs)
  (mm3) (mm3)
Band Neutrophils - -
Neutrophils 2188-7800 1700-7500
Eosinophils 40-390 40-650
Basophils 10-136 7-140
Monocytes 130-860 28-825
Lymphocytes 875-3300 1250-7000
Immature Granulocytes - -
Comments:
Refer to BD Microtainer® Tubes product sheet for detailed sample collection instructions.

A CBC must be ordered to perform a differential. Hematology instruments are automated differential screening instruments that identify WBC's by their size and staining activity. It performs a complete cell identification that includes neutrophils, lymphocytes, monocytes, basophils, eosinophils and immature granulocytes. The automated instruments count 32,000 white blood cells and statistically provide a more accurate differential count than a manual differential. All differentials will be analyzed on the instrument first. If the results fail the screening criteria, a manual differential will be performed. Pathologist approval is required to perform a manual differential on a specimen with an automated differential that passed the screening criteria.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Methodology:
Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain
CPT Code:
85025
 
See Additional Information:
Hematology Pediatric Reference Ranges