B. burgdorferi (Lyme)
| Label Mnemonic: | CLYMEAB |
| Epic code: | LAB2654 |
| Downtime form: | Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Commercial Mailout Laboratory
6240-8 RCP
356-8593
6240-8 RCP
356-8593
Specimen(s):
CSF
Collection Medium:
) |
| CSF collection kit |
Minimum:
Preferred Minimum: 3 mL CSF
Absolute Minimum: 0.5 mL CSF
With a traumatic tap CSF from tube #3 is preferred.
Absolute Minimum: 0.5 mL CSF
With a traumatic tap CSF from tube #3 is preferred.
Rejection Criteria:
Contaminated, heat-inactivated, or hemolyzed specimens.
Turn Around
Time:
1-3 days upon receipt at reference laboratory
Reference Range:
0.99 LIV: Negative - Antibody to B. burgdorferi not
detected.
1.00-1.20 LIV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greater: Positive - Probable presence of antibody to
B. burgdorferi detected.Interpretive Data:
The detection of antibodies to B. burgdorferi in
cerebrospinal fluid may indicate central nervous system infection.
However, consideration must be given to possible contamination by
blood or transfer of serum antibodies across the blood-brain
barrier.
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with immunoblot. Both IgM and IgG immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate immunoblot testing within 10 days.
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with immunoblot. Both IgM and IgG immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate immunoblot testing within 10 days.
Comments:
Note - Once this test is performed, if:
a) Negative - no further testing is done.
b) Positive or equivocal - Immunoblot testing will be performed on
the original sample upon receiving a request. Sample will be held
for 30 days only.Methodology:
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
CPT Code:
86618