Borrelia burgdorferi (Lyme), VlsE1/pepC10 Antibodies, CSF, Total by ELISA With Reflex to IgM and IgG by Immunoblot (Standard Two-Tier Testing, CSF)
| Label Mnemonic: | CLYMEAB |
| Epic code: | LAB2654 |
| Downtime form: | Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Commercial Mailout Laboratory
6240-8 RCP
356-8593
6240-8 RCP
356-8593
Specimen(s):
CSF
Collection Medium:
) |
| CSF collection kit |
Minimum:
Preferred Minimum: 6 mL CSF
Absolute Minimum: 2.5 mL CSF
With a traumatic tap CSF from tube #3 is preferred.
Rejection Criteria:
Bacterially contaminated, heat-inactivated, hemolyzed, or xanthochromic specimens.
Turn Around
Time:
1-4 days upon receipt at reference laboratory
Reference Range:
0.90 IV: Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater: Positive - VlsE1 and pepC10 antibodies to
B. burgdorferi detected.Interpretive Data:
The detection of antibodies to Borrelia burgdorferi in cerebrospinal fluid may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier. Lyme disease diagnosis in serum is recommended prior to any CSF studies.
Methodology:
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
CPT Code:
86618, 86617x2 if reflex
Note - Once this test is performed, if: a) Negative - no further testing is done. b) Positive or equivocal - Immunoblot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days only.