JC Virus (Polyomavirus) by PCR
Label Mnemonic: JCVPCR
Epic Lab Code: LAB3454
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
CSF
Collection Medium:
CSF collection kit
Minimum:
Spinal Fluid (For Detection of JC Virus Only)
0.5 mL of spinal fluid.
Turn Around Time:
3-5 days upon receipt at reference laboratory
Reference Range:
Negative

Positive results will be reported as JC virus DNA detected.
Interpretive Data:
Detection of JC virus (JCV) DNA supports the clinical diagnosis of progressive multifocal leukoencephalopathy due to JCV.
Comments:
Maintain sterility.

Clinical Information:
JC virus (JCV), a member of the genus Polyomavirus, is a small non-enveloped DNA-containing virus. Primary infection occurs in early childhood, with a prevalence of >80%. The virus is latent but can reactivate in immunosuppressed patients, especially those with AIDS.

JCV is recognized as the etiologic agent of progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system. Histologic examination of brain biopsy tissue may reveal characteristic pathologic changes localized mainly in oligodendrocytes and astrocytes. Detection of JCV DNA by PCR (target gene, large T antigen) in the cerebrospinal fluid specimens of patients with suspected PML infection has replaced the need for biopsy tissue for laboratory diagnosis. Importantly, the PCR test is specific with no cross-reaction with BK virus, a closely related polyomavirus.

Cautions:
A negative result does not rule out the possibility of JC virus (JCV) infection.

This test is not to be used as a diagnostic tool for Creutzfeldt-Jakob disease.

The reference range in cerebrospinal fluid is "negative" for this assay, although JCV DNA may be detectable in the absence of clinical symptoms in certain patient populations. However, this assay is only to be used for patients with appropriate neurological and neuroradiological features of progressive multifocal leukoencephalopathy, and is not indicated for screening asymptomatic patients.
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
CPT Code:
87798