Asparaginase Activity
| Label Mnemonic: | LASPARA |
| Epic code: | LAB8945 |
| Downtime form: | A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery |
Commercial Mailout Laboratory
6240-8 RCP
356-8593
6240-8 RCP
356-8593
Specimen(s):
Serum
Collection Medium:
 |
| Red top tube 5 mL (Clot Activator) |
Minimum:
Preferred Minimum: 2 mL serum in red top tube
Absolute Minimum: 0.5 mL serum in red top tube
Absolute Minimum: 0.5 mL serum in red top tube
Reference Range:
None. Physician established therapeutic range.
Comments:
Asparaginase is an enzyme produced by some plants, animals, and bacteria that hydrolyzes the amino acid asparagine to aspartic acid. It is commonly produced in native forms from Escherichia coli or Erwinia chrysanthemi and has a molecular weight of 138-141 kDa. PEG-modified E.coli has a MW of 145 kDa.
Test
Limitations:
Interferences: Gross hemolysis; gross lipemia; icteremia (activity assay).
Measurement of samples are made against a standard curve generated from E. coli L-asparaginase with each run. Calculations for Erwinaze® and Oncaspar® were empirically derived and utilize a correction factor that has been correlated to the E. coli L-asparaginase activity.
Measurement of samples are made against a standard curve generated from E. coli L-asparaginase with each run. Calculations for Erwinaze® and Oncaspar® were empirically derived and utilize a correction factor that has been correlated to the E. coli L-asparaginase activity.
Methodology:
Spectrophotometry/absorbance based enzyme-coupled kinetic reaction. Results are reported in IU/mL (activity assay). Traditional antigen-capture ELISA assay. Results are reported as positive or negative for antibodies (antibody assay).
CPT Code:
82657x2