Microsporidia Species, PCR
Label Mnemonic: LCMSP
Epic code: LAB8918
Downtime form: A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Commercial Mailout Laboratory
6240-8 RCP
356-8593
Specimen(s):
Stool
Collection Medium:
Sterile Container
Minimum:
Preferred Minimum: 5 g of stool in clean unpreserved stool transport vial.
Absolute Minimum: 1 g of stool
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Monday through Saturday
Turn Around Time:
2 days upon receipt at reference laboratory
Reference Range:
Negative
Interpretive Data:
A positive result indicates the presence of Enterocytozoon bieneusi and Encephalitozoon species DNA and is consistent with an active or recent infection. Since microsporidia DNA may be present in stool or urine in the absence of clinical symptoms, results should be correlated with clinical presentation.

A negative result indicates absence of detectable DNA from Enterocytozoon bieneusi and Encephalitozoon species in the specimen, but does not always rule out ongoing microsporidiosis since the organism may be present at very low levels or may be sporadic.

Other tests to consider in the evaluation of a patient presenting with acute or chronic watery diarrhea include cultures or specific assays for bacterial, viral, and parasitic pathogens.
Comments:
Useful for the detection of (em>Enterocytozoon bieneusi and Encephalitozoon species in stool and urine specimens to support the clinical diagnosis of microsporidiosis.

Cautions:
This test only detects DNA from Enterocytozoon bieneusi and Encephalitozoon species and does not detect the less common microsporidia. These 2 genera are the most common causes of intestinal and renal microsporidiosis.

While this assay is designed to detect symptomatic infection with Enterocytozoon bieneusi and Encephalitozoon species, it may detect asymptomatic carriage and should only be used for patients with a clinical history and symptoms consistent with microsporidiosis.
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
CPT Code:
87798