NPM1 Mutation Quantitative, MRD Monitoring and Interpretation

Label Mnemonic:	NPM1QNT
Epic code:	LAB8828
Downtime form:	Doctor/Provider Orders - Pathology Molecular

Molecular Pathology
6240 RCP
384-9568

Specimen(s):

Whole Blood

Specimen Instructions:

Testing requires dedicated collection tube.
DO NOT CENTRIFUGE. Store and send whole blood refrigerated.

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

5.0 mL Whole Blood in EDTA anti-coagulated pink top tube

Delivery Instructions:

Deliver to laboratory immediately after collection.

Turn Around Time:

10-14 days upon receipt in laboratory

Methodology:

The NPM1 Mutation Quantitation assay uses multiplex reverse transcription in combination with droplet digital PCR technology. The type A NPM1 duplications occur in most AML cases. The assay can reliably detect 1 tumor cell in a background of 20,000 normal cells. The limit of quantitation is 0.005% NPM1/ABL1 ratio.

CPT Code:

81310